• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IL-31 Polyclonal antibody

IL-31 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 22859-1-AP

Synonyms

IL 31, IL31, IL-31, interleukin 31

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-31 Polyclonal antibody (22859-1-AP)

    IHC staining of human tonsillitis using 22859-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-31 Polyclonal antibody (22859-1-AP)

    IHC staining of human tonsillitis using 22859-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-31 Polyclonal antibody (22859-1-AP)

    IHC staining of human tonsillitis using 22859-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-31 Polyclonal antibody (22859-1-AP)

    IHC staining of human tonsillitis using 22859-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22859-1-AP (IL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL-31 Polyclonal antibody (22859-1-AP)

    IHC staining of human small intestine using 22859-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22859-1-AP (IL31 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL-31 Polyclonal antibody (22859-1-AP)

    IHC staining of human small intestine using 22859-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22859-1-AP (IL31 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22859-1-AP is IL-31 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-31 fusion protein Ag18848
Full Name interleukin 31
Calculated Molecular Weight 164 aa, 18 kDa
GenBank Accession NumberBC132998
Gene Symbol IL31
Gene ID (NCBI) 386653
RRIDAB_2879179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-31 mRNA encodes a 164-aa precursor and a predicted 141-aa mature polypeptide, displaying an apparent molecular mass of 24 kDa in its glycosylated form. IL31 is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.

Protocols

Product Specific Protocols
IHC protocol for IL-31 antibody 22859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Rep

Interleukin-31 expression and relation to disease severity in human asthma.

Authors - Tianwen Lai
View Article
humanIHC,ELISA

World J Gastrointest Oncol

Clinical implications of interleukins-31, 32, and 33 in gastric cancer

Authors - Qing-Hua Liu
View Article