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INE1 Polyclonal antibody

INE1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21340-1-AP

Synonyms

INE1, NCRNA00010

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using INE1 Polyclonal antibody (21340-1-AP)

    WB analysis of human brain using 21340-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21340-1-AP (INE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using INE1 Polyclonal antibody (21340-1-AP)

    IHC staining of human liver using 21340-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21340-1-AP (INE1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using INE1 Polyclonal antibody (21340-1-AP)

    IHC staining of human liver using 21340-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21340-1-AP (INE1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21340-1-AP is INE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INE1 fusion protein Ag15601
Full Name inactivation escape 1 (non-protein coding)
Calculated Molecular Weight 51 aa, 5 kDa
Observed Molecular Weight 5-8 kDa
GenBank Accession NumberBC069772
Gene Symbol INE1
Gene ID (NCBI) 8552
RRIDAB_10859781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for INE1 antibody 21340-1-APDownload protocol
IHC protocol for INE1 antibody 21340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols