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Tested Applications
| Positive WB detected in | mouse uterus tissue, HepG2 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10905-1-AP targets ING3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1357 Product name: Recombinant human ING3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-92 aa of BC009777 Sequence: MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVSEFFMNAKKNKPEWREEQMASIKKDYYKALEDADEKVQLANQIYDLQHF Predict reactive species |
| Full Name | inhibitor of growth family, member 3 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC009777 |
| Gene Symbol | ING3 |
| Gene ID (NCBI) | 54556 |
| RRID | AB_2127780 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXR8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ING3 antibody 10905-1-AP | Download protocol |
| WB protocol for ING3 antibody 10905-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Clin Cancer Res Prognostic significance of nuclear ING3 expression in human cutaneous melanoma. | ||
Oncogene The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system.
| ||
Mol Cell Biol p90 RSK2 mediates antianoikis signals by both transcription-dependent and -independent mechanisms. | ||
Front Oncol Nuclear ING3 Expression Is Correlated With a Good Prognosis of Breast Cancer. | ||
Mol Cancer Res RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival. | ||
Indian J Med Res Downregulated inhibitor of growth 3 (ING3) expression during colorectal carcinogenesis. |
