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WB analysis of Jurkat using 10665-1-AP
WB result of ING5 antibody (10665-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING5 transfected Jurkat cells.
WB analysis of Jurkat using 10665-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10665-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10665-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10665-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10665-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10665-1-AP
IP result of anti-ING5 (IP:10665-1-AP, 4ug; Detection:10665-1-AP 1:700) with HEK-293 cells lysate 2560ug.
IHC staining of human colon cancer using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10665-1-AP (ING5 antibody) at dilution of 1:100 (under 25x lens).
IHC staining of human thyroid cancer using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat skin using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

ING5 Polyclonal antibody

Name: ING5 Polyclonal antibody
Brand: Proteintech
SKU: 10665-1-AP
Rating: 4.00 (1 reviews)

ING5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10665-1-AP

Publications(28)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Inhibitor of growth protein 5, p28ING5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (28)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, PC-3 cells, mouse kidney tissue, HEK-293 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human ovary tumor tissue, human thyroid cancer tissue, mouse kidney tissue, rat skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 22 publications below
IHC	See 10 publications below
IF	See 5 publications below

Product Information

10665-1-AP targets ING5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1059 Product name: Recombinant human ING5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC005370 Sequence: MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGK Predict reactive species
Full Name	inhibitor of growth family, member 5
Calculated Molecular Weight	28 kDa
Observed Molecular Weight	28-32 kDa
GenBank Accession Number	BC005370
Gene Symbol	ING5
Gene ID (NCBI)	84289
RRID	AB_2280522
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WYH8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)

Protocols

Product Specific Protocols
IHC protocol for ING5 antibody 10665-1-AP	Download protocol
IP protocol for ING5 antibody 10665-1-AP	Download protocol
WB protocol for ING5 antibody 10665-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Cell Res LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration. Authors - Yahan Wu KD Validated View Article
mouse	WB	EMBO Rep SCFJFK is functionally linked to obesity and metabolic syndrome. Authors - Lin He View Article
human	WB	Oncotarget The miR-193a-3p-regulated ING5 gene activates the DNA damage response pathway and inhibits multi-chemoresistance in bladder cancer. Authors - Yang Li View Article
human	WB	Oncotarget ING5 inhibits cancer aggressiveness via preventing EMT and is a potential prognostic biomarker for lung cancer. Authors - Feng Zhang View Article
human	IF	Oncotarget ING5 suppresses proliferation, apoptosis, migration and invasion, and induces autophagy and differentiation of gastric cancer cells: a good marker for carcinogenesis and subsequent progression. Authors - Wen-feng Gou KD Validated View Article
human	WB,IHC	Oncotarget Upregulated in Hepatitis B virus-associated hepatocellular carcinoma cells, miR-331-3p promotes proliferation of hepatocellular carcinoma cells by targeting ING5. Authors - Yiyi Cao KD Validated View Article