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WB analysis using 12057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12057-2-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12057-2-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 12057-2-AP
IP result of anti-IP6K1 (IP:12057-2-AP, 4ug; Detection:12057-2-AP 1:300) with mouse kidney tissue lysate 3000 ug.
IHC staining of human lung cancer using 12057-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12057-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12057-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12057-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12057-2-AP (IP6K1 antibody) at dilution of 1:50 (under 10x lens).

IP6K1 Polyclonal antibody

Name: IP6K1 Polyclonal antibody
Brand: Proteintech
SKU: 12057-2-AP

IP6K1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12057-2-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Synonyms

IHPK1, InsP6 kinase 1, IP6K1, KIAA0263, PiUS

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A375 cells, human liver tissue, human kidney tissue, Hela cells, HepG2 cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

12057-2-AP targets IP6K1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2687 Product name: Recombinant human IP6K1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC012944 Sequence: MCVCQTMEVGQYGKNASRAGDRGVLLEPFIHQVGGHSSMMRYDDHTVCKPLISREQRFYESLPPEMKEFTPEYKGVVSVCFEGDSDGYINLVAYPYVESETVEQDDTTEREQPRRKHSRRSLHRSGSGSDHKEEKASLSLETSESSQEAKSPKVELHSHSEVPFQMLDGNSGLSSEKISHNPWSLRCHKQQLSRMRSESKDRKLYKFLLLENVVHHFKYPCVLDLKMGTRQHGDDASAEKAARQMRKCEQSTSATLGVRVCGMQVYQLDTGHYLCRNKYYGRGLSIEGFRNALYQYLHNG Predict reactive species
Full Name	inositol hexakisphosphate kinase 1
Calculated Molecular Weight	441 aa, 50 kDa
Observed Molecular Weight	54 kDa
GenBank Accession Number	BC012944
Gene Symbol	IP6K1
Gene ID (NCBI)	9807
RRID	AB_2127529
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92551
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for IP6K1 antibody 12057-2-AP	Download protocol
IP protocol for IP6K1 antibody 12057-2-AP	Download protocol
WB protocol for IP6K1 antibody 12057-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	WB	Pediatr Res Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets Authors - Agus Suryawan View Article