Tested Applications
| Positive WB detected in | THP-1 cells, human plasma |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17342-1-AP targets CD11c in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11380 Product name: Recombinant human CD11c/Integrin alpha X protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-352 aa of BC038237 Sequence: NLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMA Predict reactive species |
| Full Name | integrin, alpha X (complement component 3 receptor 4 subunit) |
| Calculated Molecular Weight | 1169 aa, 129 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC038237 |
| Gene Symbol | CD11c |
| Gene ID (NCBI) | 3687 |
| ENSEMBL Gene ID | ENSG00000140678 |
| RRID | AB_2129787 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20702 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD11c antibody 17342-1-AP | Download protocol |
| IHC protocol for CD11c antibody 17342-1-AP | Download protocol |
| WB protocol for CD11c antibody 17342-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CD11c Polyclonal Antibody (Cat# 17342-1-AP) has 84 total citations published across more than 60 journals, including Cell, Nature Metabolism, PNAS, ACS Nano, Advanced Science, Biomaterials, and Molecular Therapy. Research fields covered include tumor immunology and immunotherapy, macrophage/dendritic cell biology, inflammation, nanomedicine and drug delivery, wound healing, and metabolic diseases. Cited applications are predominantly IHC, IF, and WB.
| Species | Application | Title |
|---|---|---|
Cell Extracellular vesicles from the lung pro-thrombotic niche drive cancer-associated thrombosis and metastasis via integrin beta 2 | ||
Cancer Commun (Lond) Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis. | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Nat Metab The antitumour effects of caloric restriction are mediated by the gut microbiome | ||
Bioact Mater Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect. | ||
Bioact Mater Light-cured millineedle platform delivers "nano-pomegranate" for combinatorial electrodynamic therapy and cuproptosis against oral carcinoma. |
