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Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Product Information
18306-1-AP targets ITM2A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | integral membrane protein 2A |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC040437 |
| Gene Symbol | ITM2A |
| Gene ID (NCBI) | 9452 |
| RRID | AB_2249412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ITM2A, also named BRICD2A and E25A, is a member of type II integral membrane (ITM2) protein family. ITM2A expression is high in osteogenic and lymphoid tissues, while both ITM2B and ITM2C are expressed in brain. There are 5 articles specifically referring to this gene in PubMed. Proteins are expected to localize in various compartments (cytoplasm, integral to membrane). No phenotype has yet been reported to our knowledge: this gene's in vivo function is yet unknown.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ITM2A antibody 18306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bone Res Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice. | ||
Blood Identification of early gene expression changes during human Th17 cell differentiation. | ||
Nutrients Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment | ||
Cell Commun Signal Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction. | ||
iScience The regenerating skeletal muscle niche drives satellite cell return to quiescence. |
