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IVNS1ABP Polyclonal antibody

IVNS1ABP Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14741-1-AP

Synonyms

ARA3, FLARA3, HSPC068, IVNS1ABP, KIAA0850, ND1, NS 1, NS1, NS1 binding protein, NS1 BP, NS1BP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of mouse heart using 14741-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of HeLa using 14741-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of HEK-293 using 14741-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Apoptosised HeLa cells using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of Apoptosised HeLa using 14741-1-AP

    Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of mouse ovary using 14741-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of mouse ovary using 14741-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using IVNS1ABP Polyclonal antibody (14741-1-AP)

    WB analysis of K-562 using 14741-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IP experiment of mouse heart using 14741-1-AP

    IP result of anti-IVNS1ABP (IP:14741-1-AP, 4ug; Detection:14741-1-AP 1:800) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of human kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of human kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of human heart using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of human heart using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of mouse kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IVNS1ABP Polyclonal antibody (14741-1-AP)

    IHC staining of mouse kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse heart tissue, Apoptosised HeLa cells, HEK-293 cells, HeLa cells, K-562 cells, mouse ovary tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14741-1-AP targets IVNS1ABP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IVNS1ABP fusion protein Ag6412
Full Name influenza virus NS1A binding protein
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC067739
Gene Symbol IVNS1ABP
Gene ID (NCBI) 10625
RRIDAB_10638784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IVNS1ABP antibody 14741-1-APDownload protocol
IHC protocol for IVNS1ABP antibody 14741-1-APDownload protocol
IP protocol for IVNS1ABP antibody 14741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Global landscape of HIV-human protein complexes.

Authors - Stefanie Jäger
View Article