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Tested Applications
| Positive WB detected in | human plasma tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20758-1-AP targets Human Ig Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14693 Product name: Recombinant human IGLC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC007782 Sequence: MAWTVLLLGLLSHCTGSGTSYVLTQPASVSVAPGQTARITCGGSNLGSKSVNWYQLRPGQAPILVVYENKERPAGIPERLSALTSEETATLTISSVVAGDEADYFCQVWDTTSQQYVFGTGTQVTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS Predict reactive species |
| Full Name | immunoglobulin lambda constant 1 (Mcg marker) |
| Calculated Molecular Weight | 233 aa, 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC007782 |
| Gene Symbol | IgG Lambda Light Chain |
| Gene ID (NCBI) | 3537 |
| RRID | AB_2878734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This antibody detects the lambda light chain of human immunoglobulin.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Human Ig Lambda Light Chain antibody 20758-1-AP | Download protocol |
| IHC protocol for Human Ig Lambda Light Chain antibody 20758-1-AP | Download protocol |
| WB protocol for Human Ig Lambda Light Chain antibody 20758-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Discov Oncol IGLC1 is an independent prognostic marker and potent therapeutic target in osteosarcoma | ||
iScience SFTSV infection is associated with transient overproliferation of monoclonal lambda-type plasma cells |
