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JMY Polyclonal antibody

JMY Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25098-1-AP

Synonyms

JMY, WHDC1L3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using JMY Polyclonal antibody (25098-1-AP)

    WB analysis of Jurkat using 25098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using JMY Polyclonal antibody (25098-1-AP)

    WB analysis of HeLa using 25098-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using JMY Polyclonal antibody (25098-1-AP)

    IP experiment of Jurkat using 25098-1-AP

    IP result of anti-JMY (IP:25098-1-AP, 4ug; Detection:25098-1-AP 1:600) with Jurkat cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using JMY Polyclonal antibody (25098-1-AP)

    IHC staining of human colon cancer using 25098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using JMY Polyclonal antibody (25098-1-AP)

    IHC staining of human brain using 25098-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using JMY Polyclonal antibody (25098-1-AP)

    IHC staining of human colon cancer using 25098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using JMY Polyclonal antibody (25098-1-AP)

    IHC staining of human brain using 25098-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JMY Polyclonal antibody (25098-1-AP)

    IF Staining of HeLa using 25098-1-AP

    Immunofluorescent analysis of HeLa cells using 25098-1-AP (JMY antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25098-1-AP is JMY Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JMY fusion protein Ag18735
Full Name junction mediating and regulatory protein, p53 cofactor
Calculated Molecular Weight 988 aa, 111 kDa
Observed Molecular Weight 111 kDa
GenBank Accession NumberBC130624
Gene Symbol JMY
Gene ID (NCBI) 133746
RRIDAB_2879895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Junction mediating and regulatory protein(JMY) is a 988 amino acid protein, which contains one WH2 domain and belongs to the JMY family. IIn nucleus, JMY acts both a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics in response to cellular stress. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Due to alternative splicing, several isoforms of JMY are produced, and these various isoforms have different influencing affects on p53 activation, with some isoforms markedly enhancing p53 responses compared to the other splicing variants.

Protocols

Product Specific Protocols
WB protocol for JMY antibody 25098-1-APDownload protocol
IHC protocol for JMY antibody 25098-1-APDownload protocol
IF protocol for JMY antibody 25098-1-APDownload protocol
IP protocol for JMY antibody 25098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS Genet

WHIMP links the actin nucleation machinery to Src-family kinase signaling during protrusion and motility.

Authors - Shail Kabrawala
View Article