Kir6.1 Polyclonal antibody

Kir6.1 Polyclonal Antibody for WB, ELISA

Cat No. 14954-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

KCNJ8, Kir6.1, uKATP 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis using 14954-1-AP
Unboiled HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 14954-1-AP (Kir6.1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

unboiled HEK-293 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14954-1-AP targets Kir6.1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6792 Product name: Recombinant human KCNJ8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 181-424 aa of BC000544 Sequence: TAQAHRRAETLIFSRHAVIAVRNGKLCFMFRVGDLRKSMIISASVRIQVVKKTTTPEGEVVPIHQLDIPVDNPIESNNIFLVAPLIICHVIDKRSPLYDISATDLANQDLEVIVILEGVVETTGITTQARTSYIAEEIQWGHRFVSIVTEEEGVYSVDYSKFGNTVKVAAPRCSARELDEKPSILIQTLQKSELSHQNSLRKRNSMRRNNSMRRNNSIRRNNSSLMVPKVQFMTPEGNQNTSES Predict reactive species
Full Name	potassium inwardly-rectifying channel, subfamily J, member 8
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	51 kDa
GenBank Accession Number	BC000544
Gene Symbol	Kir6.1
Gene ID (NCBI)	3764
RRID	AB_2130768
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15842
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for Kir6.1 antibody 14954-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB,IF	Brain Behav Immun Opening K(ATP) channels induces inflammatory tolerance and prevents chronic pain Authors - Cheng Qian View Article
rat	WB	Pharmacol Res Perspect Tetramisole is a new IK1 channel agonist and exerts IK1 -dependent cardioprotective effects in rats. Authors - Qinghua Liu View Article