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WB analysis of mouse skeletal muscle using 21111-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21111-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 21111-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 21111-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 21111-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 21111-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 21111-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 21111-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 21111-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCTD14 Polyclonal antibody

Name: KCTD14 Polyclonal antibody
Brand: Proteintech
SKU: 21111-1-AP

KCTD14 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21111-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

BTB/POZ domain-containing protein KCTD14

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, mouse brain tissue, mouse small intestine tissue
Positive IHC detected in	human stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21111-1-AP targets KCTD14 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14265 Product name: Recombinant human KCTD14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-225 aa of BC001929 Sequence: MSTVVELNVGGEFHTTTLGTLRKFPGSKLAEMFSSLAKASTDAEGRFFIDRPSTYFRPILDYLRTGQVPTQHIPEVYREAQFYEIKPLVKLLEDMPQIFGEQVSRKQFLLQVPGYSENLELMVRLARAEAITARKSSVLVCLVETEEQDAYYSEVLCFLQDKKMFKSVVKFGPWKAVLDNSDLMHCLEMDIKAQGYKVFSKFYLTYPTKRNEFHFNIYSFTFTWW Predict reactive species
Full Name	potassium channel tetramerisation domain containing 14
Calculated Molecular Weight	225 aa, 26 kDa
Observed Molecular Weight	30 kDa
GenBank Accession Number	BC001929
Gene Symbol	KCTD14
Gene ID (NCBI)	65987
RRID	AB_10733343
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BQ13
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCTD14, or potassium channel tetramerization domain containing 14, is a member of the KCTD family of proteins. This family consists of 25 members in humans, many of which are only partially characterized . KCTD14, like other members of the KCTD family, contains a conserved domain known as the BTB (Broad complex, Tramtrak, and Bric-a-brac)/POZ (poxvirus zinc finger) domain, which is crucial for protein oligomerization and establishing protein-protein interactions. KCTD14 has been implicated in various biological functions, and recent research suggests a potential role in cancer. Although not as extensively studied as some other KCTD members, KCTD14 has been noted in database analyses to potentially play a protumor role in ovarian cancer. Specifically, the COSMIC database reports a copy number variation (CNV) gain in 4.5% of ovarian cancers, and the GENT2 database indicates a fold change (FC) of 1.5 in expression, with a p-value less than 0.001, suggesting a possible association with ovarian cancer.