KEAP1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | IF, IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, monkey, mouse, rat |
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human lung cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
IP = Immunoprecipitation
IP result of anti-KEAP1 (10503-2-AP for IP and Detection) with mouse skeletal muscle tissue lysate.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10503-2-AP(KEAP1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer using 10503-2-AP(KEAP1 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lung cancer using 10503-2-AP(KEAP1 Antibody) at Dilution 1:100 (under 40x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | KEAP1 fusion protein ag0779 | Full name | kelch-like ECH-associated protein 1 |
|
Calculated molecular weight |
624aa,70kd |
Observed molecular
weight |
60-70 kDa |
|
GenBank accession number |
BC002930 | Gene ID (NCBI) | 9817 |
| Gene symbol | KEAP1 | Synonyms | INrf2; KIAA0132; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454 |
KEAP1, also named as INRF2, KIAA0132 and KLHL19, is part of a multiprotein complex that contains the CUL3-ROC1 ubiquitin ligase, which can ubiquitinate the N-terminal domain of NRF2[PMID: 20173742]. Two molecules of KEAP1 bind to two distinct sites in the N-terminal region of NRF2, the ETGE and DLG sites, which affect the KEAP1-NRF2 interaction and/or its physiological consequences[PMID: 22215675]. KEAP1 retains NFE2L2/NRF2 in the cytosol. It functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1[PMID: 20427290]. It also retains BPTF in the cytosol. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human KEAP1. |
