KIAA0325 Antibody
Rabbit Polyclonal
Catalog number: 12345-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat, zebrafish; other species not tested. |
| Cited species | human, Zebrafish |
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human breast cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP(KIAA0325 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human breast cancer using 12345-1-AP(KIAA0325 Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | KIAA0325 fusion protein ag2999 | Full name | dynein, cytoplasmic 1, heavy chain 1 |
|
Calculated molecular weight |
4646aa,532kd |
Observed molecular
weight |
532kd |
|
GenBank accession number |
BC021297 | Gene ID (NCBI) | 1778 |
| Gene symbol | DYNC1H1 | Synonyms | DHC1; DHC1a; DKFZp686P2245; DNCH1; DNCL; DNECL; DYHC; Dnchc1; HL-3; KIAA0325; p22 |
Background
Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients.. |
