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MARF1 Polyclonal antibody

MARF1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25233-1-AP

Synonyms

MARF1, A 362G6.1, KIAA0430, Limkain b1, LKAP

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MARF1 Polyclonal antibody (25233-1-AP)

    WB analysis of HEK-293 using 25233-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25233-1-AP (KIAA0430 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MARF1 Polyclonal antibody (25233-1-AP)

    WB analysis of HeLa using 25233-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25233-1-AP (KIAA0430 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MARF1 Polyclonal antibody (25233-1-AP)

    IP experiment of HeLa using 25233-1-AP

    IP result of anti-MARF1 (IP:25233-1-AP, 5ug; Detection:25233-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MARF1 Polyclonal antibody (25233-1-AP)

    IHC staining of human small intestine using 25233-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MARF1 Polyclonal antibody (25233-1-AP)

    IHC staining of human ovary using 25233-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MARF1 Polyclonal antibody (25233-1-AP)

    IHC staining of human ovary using 25233-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MARF1 Polyclonal antibody (25233-1-AP)

    IHC staining of human small intestine using 25233-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25233-1-AP (KIAA0430 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MARF1 Polyclonal antibody (25233-1-AP)

    IF Staining of HEK-293 using 25233-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 25233-1-AP (KIAA0430 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25233-1-AP is MARF1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARF1 fusion protein Ag18905
Full Name KIAA0430
Calculated Molecular Weight 1742 aa, 193 kDa
Observed Molecular Weight 192 kDa
GenBank Accession NumberBC137165
Gene Symbol KIAA0430
Gene ID (NCBI) 9665
RRIDAB_2879977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARF1, also known as KIAA0430, or LKAP, which is expressed in peroxisome. MARF1 is essential regulator of oogenesis and required for female meiotic progression to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. MARF1 probably acts via some RNA metabolic process and is equivalent to the piRNA system in males, which mediates the repression of transposable elements during meiosis by forming complexes composed of RNAs and governs the methylation and subsequent repression of transposons.

Protocols

Product Specific Protocols
WB protocol for MARF1 antibody 25233-1-APDownload protocol
IHC protocol for MARF1 antibody 25233-1-APDownload protocol
IF protocol for MARF1 antibody 25233-1-APDownload protocol
IP protocol for MARF1 antibody 25233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

Gradient Boosting Machine Learning Model for Defective Endometrial Receptivity Prediction by Macrophage-Endometrium Interaction Modules.

Authors - Bohan Li
View Article