Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | COS-7 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14615-1-AP targets KIF17 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6217 Product name: Recombinant human KIF17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC065927 Sequence: MASEAVKVVVRCRPMNQRERELRCQPVVTVDCARAQCCIQNPGAADEPPKQFTFDGAYHVDHVTEQIYNEIAYPLVEGVTEGYNGTIFAYGQTGSGKSFTMQGLPDPPSQRGIIPRAFEHVFESVQCAENTKFLVRASYLEIYNEDVRDLLGADTKQKLELKEHPEKGVYVKGLSMHTVHSVAQCEHIMETGWKNRSVGYTLMNKDSSRSHSIFTISIEMSAVDERGKDHLRAGKLNLVDLAGSERQSKTGATGERLKEATKINLSLSALGNVISALVDGRCKHVPYRDSKLTRLLQDSLGGNTKTLMVACLSPADNNYDETLSTLRYANRAKNIRNKPRINEDPKDALLR Predict reactive species |
| Full Name | kinesin family member 17 |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | BC065927 |
| Gene Symbol | KIF17 |
| Gene ID (NCBI) | 57576 |
| RRID | AB_2131278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2E2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIF17 is a kinesin superfamily member, encoding a neuron-specific molecular motor. In neurons, KIF17 is responsible for the specialized transport of the N-methyl-D-aspartate receptor NR2B subunit, the glutamate receptor GluR5, and the potassium channel Kv4.2 from the cell body to the dendrites(PMID: 23762210).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIF17 antibody 14615-1-AP | Download protocol |
| IP protocol for KIF17 antibody 14615-1-AP | Download protocol |
| WB protocol for KIF17 antibody 14615-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIF17 polyclonal antibody (Cat# 14615-1-AP) has 4 total citations published in Cancer Letters, Developmental Cell, Neuroscience Bulletin, and Journal of Biological Chemistry. Associated research fields include breast cancer metastasis, photoreceptor cilia biology, epilepsy and NMDA receptor regulation, and protein structure related to Carpenter Syndrome. Cited applications span WB, IHC, IF, IP, and CoIP in human and mouse species.
| Species | Application | Title |
|---|---|---|
Cancer Lett KIF17 maintains the epithelial phenotype of breast cancer cells and curbs tumour metastasis
| ||
Dev Cell ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia. | ||
Neurosci Bull KIF17 Modulates Epileptic Seizures and Membrane Expression of the NMDA Receptor Subunit NR2B. | ||
J Biol Chem Structural basis for Rab23 activation and a loss-of-function mutation in Carpenter Syndrome |
