Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IHC detected in | human colon cancer, human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19245-1-AP targets KIF18A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6166 Product name: Recombinant human KIF18A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-279 aa of BC048347 Sequence: MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTTNQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGT Predict reactive species |
| Full Name | kinesin family member 18A |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC048347 |
| Gene Symbol | KIF18A |
| Gene ID (NCBI) | 81930 |
| RRID | AB_10598464 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NI77 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIF18A belongs to the Kinesin family. KIF18A binds to microtubules and moves along them using energy from ATP hydrolysis. KIF18A is related to the localization of membrane organelles and protein complexes, as well as the maintenance of microtubule dynamics and mitosis. KIF18A expression is low in normal tissues and high in solid tumors, including breast cancer, hepatocellular carcinoma and lung adenocarcinoma, colorectal cancer, renal cell carcinoma, and head and neck squamous cell carcinoma. The molecular weight of KIF18A is 102 kDa (PMID: 36830695).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIF18A antibody 19245-1-AP | Download protocol |
| WB protocol for KIF18A antibody 19245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIF18A antibody (Cat# 19245-1-AP) has 18 total citations published across 17 journals, including Cell Death & Differentiation, PNAS, Cell Mol Life Sci, Oncotarget, Sci Rep, PLoS One, and Frontiers in Genetics. Research fields span mitotic spindle biology, cancer (hepatocellular carcinoma, lung adenocarcinoma, glioma, prostate cancer, renal carcinoma, cholangiocarcinoma, colorectal cancer), liver fibrosis, and oocyte meiosis/egg aneuploidy. Applications include WB, IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Cell Death Differ USP21-mediated deubiquitylation stimulates NuMA recruitment to the cell cortex to promote mitotic spindle orientation. | ||
Proc Natl Acad Sci U S A Maternal genetic variants in kinesin motor domains prematurely increase egg aneuploidy | ||
Cell Mol Life Sci KIF18A inactivates hepatic stellate cells and alleviates liver fibrosis through the TTC3/Akt/mTOR pathway | ||
Biomolecules Comprehensive Pan-Cancer Analysis of KIF18A as a Marker for Prognosis and Immunity | ||
Oncotarget High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma. | ||
