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Tested Applications
| Positive WB detected in | mouse lung tissue, mouse kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 9 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
10125-2-AP targets KIFC3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0172 Product name: Recombinant human KIFC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 73-314 aa of BC001211 Sequence: ARSAARPALAQCRALSVDWAGPGSPHGLYLTLQVEHLKEKLISQAQEVSRLRSELGGTDLEKHRDLLMVENERLRQEMRRCEAELQELRTKPAGPCPGCEHSQESAQLRDKLSQLQLEMAESKGMLSELNLEVQQKTDRLAEVELRLKDCLAEKAQEEERLSRRLRDSHETIASLRAQSPPVKYVIKTVEVESSKTKQALSESQARNQHLQEQVAMQRQVLKEMEQQLQSSHQLTARLRAQI Predict reactive species |
| Full Name | kinesin family member C3 |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 80-85 kDa, 95-100 kDa |
| GenBank Accession Number | BC001211 |
| Gene Symbol | KIFC3 |
| Gene ID (NCBI) | 3801 |
| RRID | AB_2296476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIFC3 antibody 10125-2-AP | Download protocol |
| IP protocol for KIFC3 antibody 10125-2-AP | Download protocol |
| WB protocol for KIFC3 antibody 10125-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner | ||
EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
| ||
Mol Biol Cell Kif5B and Kifc1 interact and are required for motility and fission of early endocytic vesicles in mouse liver. | ||
World J Gastrointest Oncol KIFC3 promotes proliferation, migration and invasion of esophageal squamous cell carcinoma cells by activating EMT and β-catenin signaling
| ||
Biochim Biophys Acta The iron-regulated metastasis suppressor, Ndrg-1: identification of novel molecular targets. |
