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KLHDC5 Polyclonal antibody

KLHDC5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24847-1-AP

Synonyms

kelch domain containing 5, KIAA1340, KLHDC5, KLHL42

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KLHDC5 Polyclonal antibody (24847-1-AP)

    WB analysis of HeLa using 24847-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KLHDC5 Polyclonal antibody (24847-1-AP)

    WB analysis of mouse brain using 24847-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KLHDC5 Polyclonal antibody (24847-1-AP)

    IHC staining of human kidney using 24847-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KLHDC5 Polyclonal antibody (24847-1-AP)

    IHC staining of human kidney using 24847-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KLHDC5 Polyclonal antibody (24847-1-AP)

    IF Staining of HeLa using 24847-1-AP

    Immunofluorescent analysis of HeLa cells using 24847-1-AP (KLHDC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24847-1-AP is KLHDC5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHDC5 fusion protein Ag20448
Full Name kelch domain containing 5
Calculated Molecular Weight 505 aa, 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC137116
Gene Symbol KLHDC5
Gene ID (NCBI) 57542
RRIDAB_2879754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHDC5 also named as kelch domain containing 5 or KLHL42 is a 505 amino acid protein, which contains one BTB domain and six Kelch repeats. KLHDC5 localizes in cytoplasm and may involve in microtubule dynamics throughout mitosis.

Protocols

Product Specific Protocols
WB protocol for KLHDC5 antibody 24847-1-APDownload protocol
IHC protocol for KLHDC5 antibody 24847-1-APDownload protocol
IF protocol for KLHDC5 antibody 24847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Acta Pharmacol Sin

Satellite cell-derived exosome-mediated delivery of microRNA-23a/27a/26a cluster ameliorates the renal tubulointerstitial fibrosis in mouse diabetic nephropathy

Authors - Jia-Ling Ji
View Article