KLHL13 Polyclonal antibody

KLHL13 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15612-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

BKLHD2, kelch like 13 (Drosophila), Kelch like protein 13, KIAA1309, KLHL13

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of Jurkat using 15612-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15612-1-AP (KLHL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 15612-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15612-1-AP (KLHL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 15612-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skeletal muscle using 15612-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skeletal muscle using 15612-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells
Positive IHC detected in	mouse skeletal muscle tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15612-1-AP targets KLHL13 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8003 Product name: Recombinant human KLHL13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 487-655 aa of BC008729 Sequence: GGVMYISGGITHDTFQKELMCFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSDYDDVLSCEYYSPILDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPDKDEWHKVFDLPESLGGIRACTLTVFPPEETTPSPSRESPLSAP Predict reactive species
Full Name	kelch-like 13 (Drosophila)
Calculated Molecular Weight	74 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC008729
Gene Symbol	KLHL13
Gene ID (NCBI)	90293
RRID	AB_2130739
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9P2N7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for KLHL13 antibody 15612-1-AP	Download protocol
WB protocol for KLHL13 antibody 15612-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB,IF	Sci Rep RHOBTB2 enhances cell proliferation of acute myeloid leukemia by modulating Hippo-YAP1 signaling and dependent of KLHL13. Authors - Yao Liu View Article