Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 14849-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14849-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 14849-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14849-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 14849-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14849-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14849-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14849-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 14849-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14849-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 14849-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14849-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 14849-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 14849-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 14849-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 14849-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 14849-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14-Specific antibody (14849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

KLHL14-Specific Polyclonal antibody

Name: KLHL14-Specific Polyclonal antibody
Brand: Proteintech
SKU: 14849-1-AP

KLHL14-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14849-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse heart tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse ovary tissue, HepG2 cells, rat brain tissue
Positive IHC detected in	human heart tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below

Product Information

14849-1-AP targets KLHL14-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	kelch-like 14 (Drosophila)
Calculated Molecular Weight	70 kDa
Observed Molecular Weight	39-45 kDa
GenBank Accession Number	NM_020805
Gene Symbol	KLHL14
Gene ID (NCBI)	57565
RRID	AB_2131171
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9P2G3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.

Protocols

Product Specific Protocols
IF protocol for KLHL14-Specific antibody 14849-1-AP	Download protocol
IHC protocol for KLHL14-Specific antibody 14849-1-AP	Download protocol
WB protocol for KLHL14-Specific antibody 14849-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Int J Clin Exp Pathol Kelch-like protein 14 promotes proliferation and migration of ovarian cancer cells. Authors - Zheng Chen KD Validated View Article