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  • Featured Product
  • KD/KO Validated

KLHL21 Polyclonal antibody

KLHL21 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16952-1-AP

Synonyms

kelch like 21 (Drosophila), Kelch like protein 21, KIAA0469, KLHL21

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using KLHL21 Polyclonal antibody (16952-1-AP)

    WB analysis of A431 using 16952-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KLHL21 Polyclonal antibody (16952-1-AP)

    WB analysis of HeLa using 16952-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using KLHL21 Polyclonal antibody (16952-1-AP)

    IP experiment of HeLa using 16952-1-AP

    IP result of anti-KLHL21 (IP:16952-1-AP, 3ug; Detection:16952-1-AP 1:300) with HeLa cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KLHL21 Polyclonal antibody (16952-1-AP)

    IHC staining of human liver cancer using 16952-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KLHL21 Polyclonal antibody (16952-1-AP)

    IHC staining of human brain using 16952-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KLHL21 Polyclonal antibody (16952-1-AP)

    IHC staining of human brain using 16952-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KLHL21 Polyclonal antibody (16952-1-AP)

    IHC staining of human liver cancer using 16952-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inA431 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16952-1-AP targets KLHL21 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL21 fusion protein Ag10582
Full Name kelch-like 21 (Drosophila)
Calculated Molecular Weight 597 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC034039
Gene Symbol KLHL21
Gene ID (NCBI) 9903
RRIDAB_2265294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KLHL21 antibody 16952-1-APDownload protocol
IHC protocol for KLHL21 antibody 16952-1-APDownload protocol
IP protocol for KLHL21 antibody 16952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Breast Cancer Res Treat

Tumor-promoting mechanisms of macrophage-derived extracellular vesicles-enclosed microRNA-660 in breast cancer progression.

Authors - Changchun Li
  • KD Validated
View Article