Filter: AllWBIHC

WB analysis of Jurkat using 25224-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 25224-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 25224-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 25224-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 25224-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 25224-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 25224-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 25224-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 10x lens).

KLHL28 Polyclonal antibody

Name: KLHL28 Polyclonal antibody
Brand: Proteintech
SKU: 25224-1-AP

KLHL28 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25224-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

BTBD5, kelch like 28 (Drosophila), Kelch like protein 28, KLHL28

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, mouse brain tissue
Positive IHC detected in	human liver tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25224-1-AP targets KLHL28 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18665 Product name: Recombinant human KLHL28 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 222-571 aa of BC121009 Sequence: NSVRLPLLSVKFLTRLYEANHLIRDDRTCKHLLNEALKYHFMPEHRLSHQTVLMTRPRCAPKVLCAVGGKSGLFACLDSVEMYFPQNDSWIGLAPLNIPRYEFGICVLDQKVYVIGGIATNVRPGVTIRKHENSVECWNPDTNTWTSLERMNESRSTLGVVVLAGELYALGGYDGQSYLQSVEKYIPKIRKWQPVAPMTTTRSCFAAAVLDGMIYAIGGYGPAHMNSVERYDPSKDSWEMVASMADKRIHFGVGVMLGFIFVVGGHNGVSHLSSIERYDPHQNQWTVCRPMKEPRTGVGAAVIDNYLYVVGGHSGSSYLNTVQKYDPISDTWLDSAGMIYCRCNFGLTAL Predict reactive species
Full Name	kelch-like 28 (Drosophila)
Calculated Molecular Weight	571 aa, 64 kDa
Observed Molecular Weight	64 kDa
GenBank Accession Number	BC121009
Gene Symbol	KLHL28
Gene ID (NCBI)	54813
RRID	AB_2879972
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NXS3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHL28 also named as BTBD5 or Kelch like protein 28 is a 571 amino acid protein, which contains one BTB domain and six kelch repeats. KLHL28 localizes in the cytoplasm and belongs to BTB/kelch-associated proteins family. KLHL28 may play a role in organizing the actin cytoskeleton of the brain cells. KLHL28 is expressed as two isoforms produced by alternative splicing.