Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21981-1-AP targets KLHL34 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.
| Tested Reactivity | mouse, rat, human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16001 Product name: Recombinant human KLHL34 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 295-644 aa of BC109031 Sequence: IEEVAAPQRAARGQVAAPEPEEEEEELEEEEEEEEWELTQNVVAFDVYNHRWRSLTQLPTPLLGHSVCTAGNFLFVLGGESPSGSASSPLADDSRVVTAQVHRYDPRFHAWTEVPAMREARAHFWCGAVGERLLAVGGLGAGGEVLASVEMYDLRRDRWTAAGALPRALHGHAGAVGDRGVVYISGGKAGRGEGGASSLRDLYVLGPEEQVWSKKAPMGTARFGHHMAVLRGAVFAFLGRYEPFSEIERYDPGADQWTRLRPLPYDRFCYGLAVVEETALLLGGLKWRDSRQVPTRNVVGYDLDLDRWEDIGCALPWAWSGLRCAVLQLAEGGDDEREGEVGEALDLVLG Predict reactive species |
| Full Name | kelch-like 34 (Drosophila) |
| Calculated Molecular Weight | 644 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC109031 |
| Gene Symbol | KLHL34 |
| Gene ID (NCBI) | 257240 |
| RRID | AB_2878959 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N239 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KLHL34 is a member of the Kelch-like (KLHL) gene family, which encodes a group of proteins that generally possess a BTB/POZ domain, a BACK domain and 5-6 Kelch motifs. KLHL genes are responsible for several Mendelian diseases and have been associated with cancer. The molecular mass of KLHL34 is 71 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KLHL34 antibody 21981-1-AP | Download protocol |
| WB protocol for KLHL34 antibody 21981-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
