KLHL35 Polyclonal antibody

KLHL35 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21199-1-AP

Synonyms

26597, kelch like 35 (Drosophila), Kelch like protein 35, KLHL35



Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21199-1-AP is KLHL35 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL35 fusion protein Ag15558
Full Name kelch-like 35 (Drosophila)
Calculated Molecular Weight 363 aa, 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC042952
Gene Symbol KLHL35
Gene ID (NCBI) 283212
RRIDAB_10693685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KLHL35 antibody 21199-1-APDownload protocol
IHC protocol for KLHL35 antibody 21199-1-APDownload protocol
IF protocol for KLHL35 antibody 21199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols