KNG1 Antibody
Rabbit Polyclonal
Catalog number: 11926-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, A2780 cells, COLO 320 cells, HeLa cells, MCF7 cells |
| Positive IHC detected in | human endometrial cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP(KNG1 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human endometrial cancer using 11926-1-AP(KNG1 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human endometrial cancer using 11926-1-AP(KNG1 Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using KNG1 antibody 11926-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | KNG1 fusion protein ag2574 | Full name | kininogen 1 |
|
Calculated molecular weight |
644aa,72kd |
Observed molecular
weight |
60kd or 47kd |
|
GenBank accession number |
BC060039 | Gene ID (NCBI) | 3827 |
| Gene symbol | KNG1 | Synonyms | BDK; KNG |
Background
Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein. 11926-1-AP was generated against N-terminal 300 aa of HMW kininogen. It can bind both HMW and LMW kiniogen. |
