Tested Applications
| Positive WB detected in | HeLa cells, A2780 cells, COLO 320 cells, MCF-7 cells, mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11926-1-AP targets Kininogen 1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2574 Product name: Recombinant human KNG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-374 aa of BC060039 Sequence: ESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRMTYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLG Predict reactive species |
| Full Name | kininogen 1 |
| Calculated Molecular Weight | 644 aa, 72 kDa |
| Observed Molecular Weight | 48 kDa, 72 kDa |
| GenBank Accession Number | BC060039 |
| Gene Symbol | Kininogen 1 |
| Gene ID (NCBI) | 3827 |
| RRID | AB_2133403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01042 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein. 11926-1-AP was generated against N-terminal 300 aa of HMW kininogen. It can bind both HMW and LMW kiniogen.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Kininogen 1 antibody 11926-1-AP | Download protocol |
| IHC protocol for Kininogen 1 antibody 11926-1-AP | Download protocol |
| WB protocol for Kininogen 1 antibody 11926-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Kininogen 1 (KNG1) Polyclonal Antibody (Cat# 11926-1-AP) has 12 citations across journals including NPJ Aging, EBioMedicine, Hypertension, J Neurooncol, Neuroscience, Behav Brain Res, PLoS One, and others. Research fields span skin aging, hepatitis B infection, hypertension, vitiligo, tumor progression, neuroinflammation, stroke, depression biomarkers, sleep deprivation, spinal cord injury, polycystic ovary syndrome, and renal protection. Applications cited include WB, IHC, IF, and CoIP in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
NPJ Aging Decoding skin aging: the role of KNG1 in collagen and elastic fibre degradation. | ||
EBioMedicine An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradation | ||
J Ethnopharmacol Rubia cordifolia L. extract ameliorates vitiligo by inhibiting the CXCL10/CXCL9/STAT1 signaling pathway | ||
J Neurooncol Multiomics reveals the mechanism of KNG1 in promoting tumor progression in adamantinomatous craniopharyngioma. | ||
Neuroscience Scalp acupuncture alleviates remote hippocampal damage in MCAO rats by inhibiting neuroinflammation: A TMT-based proteomics analysis |
