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  • KD/KO Validated

KPNA4 Polyclonal antibody

KPNA4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12463-1-AP

Synonyms

Importin alpha Q1, Importin subunit alpha 4, IPOA3, Karyopherin subunit alpha 4, KPNA4, QIP1, SRP3



Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, Jurkat cells, mouse testis tissue, rat testis tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12463-1-AP targets KPNA4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KPNA4 fusion protein Ag3133
Full Name karyopherin alpha 4 (importin alpha 3)
Calculated Molecular Weight 521 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC034493
Gene Symbol KPNA4
Gene ID (NCBI) 3840
RRIDAB_2133821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

Protocols

Product Specific Protocols
WB protocol for KPNA4 antibody 12463-1-APDownload protocol
IHC protocol for KPNA4 antibody 12463-1-APDownload protocol
IF protocol for KPNA4 antibody 12463-1-APDownload protocol
IP protocol for KPNA4 antibody 12463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Virol Sin

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Authors - Yisha Zhuo
humanWB

Oncogene

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

Authors - Kai Li
  • KD Validated
mouseWB

J Neurosci

The subcellular distribution of HDAC1 in neurotoxic conditions is dependent on serine phosphorylation.

Authors - Yunjiao Zhu
mouseWB

Exp Gerontol

Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.

Authors - Shama R Iyer
humanWB

Tumour Biol

Reanalysis of microRNA expression profiles identifies novel biomarkers for hepatocellular carcinoma prognosis.

Authors - Zhengqiang Wang
humanWB

Viruses

Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2

Authors - Anna Nakanishi