KPNA5 Polyclonal antibody

KPNA5 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13963-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Synonyms

Importin subunit alpha 6, IPOA6, Karyopherin subunit alpha 5, KPNA5, SRP6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIP

WB analysis of Jurkat using 13963-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13963-1-AP (KPNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 13963-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13963-1-AP (KPNA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of Jurkat using 13963-1-AP
IP result of anti-KPNA5 (IP:13963-1-AP, 4ug; Detection:13963-1-AP 1:2000) with Jurkat cells lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	Jurkat cells
Positive IP detected in	Jurkat cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

13963-1-AP targets KPNA5 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4920 Product name: Recombinant human KPNA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC047409 Sequence: MDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKRRNVYLPRNDESMLESPIQDPDISSTVPIPEEEVVTTDMVQMIFSNNADQQLTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCTLQFEAAWALTNIASGTFLHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEILPPLLELLTNSNRLTTTR Predict reactive species
Full Name	karyopherin alpha 5 (importin alpha 6)
Calculated Molecular Weight	60 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC047409
Gene Symbol	KPNA5
Gene ID (NCBI)	3841
RRID	AB_2133847
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15131
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IP protocol for KPNA5 antibody 13963-1-AP	Download protocol
WB protocol for KPNA5 antibody 13963-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human,rat	WB	Diabetologia Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function. Authors - Mu Zhang View Article
human	WB	Virol Sin African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway. Authors - Yisha Zhuo View Article
human	WB	J Immunol Cutting edge: tubulin α functions as an adaptor in NFAT-importin β interaction. Authors - Ishiguro Kazuhiro K View Article
human	WB	PLoS One An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine. Authors - Echeverría Pablo C PC View Article
human	WB	J Biol Chem The nuclear transportation of CHRONO regulates the circadian rhythm Authors - Qin Zhou View Article
	WB,CoIP	J Neurosci miR-153 prevents NRF2 nuclear translocation to drive hypoperfusion-related cognitive deficits by targeting KPNA5 Authors - Yang Qu View Article