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KRI1 Polyclonal antibody

KRI1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 16243-1-AP

Synonyms

KRI1, KRI1 homolog (S. cerevisiae), Protein KRI1 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KRI1 Polyclonal antibody (16243-1-AP)

    WB analysis of HEK-293 using 16243-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KRI1 Polyclonal antibody (16243-1-AP)

    WB analysis of HEK-293 using 16243-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KRI1 Polyclonal antibody (16243-1-AP)

    WB analysis of HepG2 using 16243-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using KRI1 Polyclonal antibody (16243-1-AP)

    WB analysis of HeLa using 16243-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KRI1 Polyclonal antibody (16243-1-AP)

    WB analysis of HeLa using 16243-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KRI1 Polyclonal antibody (16243-1-AP)

    WB analysis of HepG2 using 16243-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KRI1 Polyclonal antibody (16243-1-AP)

    IF Staining of HepG2 using 16243-1-AP

    Immunofluorescent analysis of HepG2 cells using 16243-1-AP (KRI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16243-1-AP is KRI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRI1 fusion protein Ag9171
Full Name KRI1 homolog (S. cerevisiae)
Calculated Molecular Weight 709 aa, 80 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009876
Gene Symbol KRI1
Gene ID (NCBI) 65095
RRIDAB_2878235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KRI1 antibody 16243-1-APDownload protocol
IF protocol for KRI1 antibody 16243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols