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Cytokeratin 17 Polyclonal antibody

Cytokeratin 17 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 22230-1-AP

Synonyms

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    WB analysis of HeLa using 22230-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    WB analysis of MCF-7 using 22230-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    IHC staining of human cervical cancer using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    IHC staining of human cervical cancer using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    IF Staining of HeLa using 22230-1-AP

    Immunofluorescent analysis of HeLa cells using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22230-1-AP is Cytokeratin 17 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 17 fusion protein Ag17575
Full Name keratin 17
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000159
Gene Symbol KRT17
Gene ID (NCBI) 3872
RRIDAB_2879040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
IHC protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
IF protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols