KRT34 Antibody
Rabbit Polyclonal
Catalog number: 20449-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | A431 cells, A375 cells, HeLa cells, HepG2 cells, mouse skin tissue, L02 cells |
| Positive IHC detected in | human skin |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
A431 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP(KRT34 Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human skin using 20449-1-AP(KRT34 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skin using 20449-1-AP(KRT34 Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using KRT34 antibody 20449-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | KRT34 fusion protein ag14295 | Full name | keratin 34 |
|
Calculated molecular weight |
436aa,49kd |
Observed molecular
weight |
43kd,49kd |
|
GenBank accession number |
BC041070 | Gene ID (NCBI) | 3885 |
| Gene symbol | KRT34 | Synonyms | HA4; Ha-4; KRTHA4; hHa4 |
Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). KRT 34 is a type I keratin. |
