- Featured Product
- KD/KO Validated
KRT80 Polyclonal antibody
KRT80 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,IHC,ELISA
Conjugate
Unconjugated
Cat no : 16835-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, human skeletal muscle tissue |
| Positive IHC detected in | human skin cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3600 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 9 publications below |
| IHC | See 5 publications below |
| IF | See 6 publications below |
Product Information
16835-1-AP targets KRT80 in WB,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KRT80 fusion protein Ag10498 |
| Full Name | keratin 80 |
| Calculated Molecular Weight | 422 aa, 47 kDa |
| Observed Molecular Weight | 47 kDa, 50 kDa |
| GenBank Accession Number | BC065180 |
| Gene Symbol | KRT80 |
| Gene ID (NCBI) | 144501 |
| RRID | AB_1851273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KRT80 antibody 16835-1-AP | Download protocol |
| IHC protocol for KRT80 antibody 16835-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine. | ||
Cell Discov Single-cell atlas of keratoconus corneas revealed aberrant transcriptional signatures and implicated mechanical stretch as a trigger for keratoconus pathogenesis. | ||
Int J Mol Sci Keratin Profiling by Single-Cell RNA-Sequencing Identifies Human Prostate Stem Cell Lineage Hierarchy and Cancer Stem-Like Cells. | ||
Cell Death Dis Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway. | ||
Cell Cycle MiR-4268 suppresses gastric cancer genesis through inhibiting keratin 80.
| ||
Cancer Biol Ther The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer
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