Tested Applications
| Positive WB detected in | NCCIT cells |
| Positive IHC detected in | human ovary cancer tissue, human ovary tumor tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NCCIT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21528-1-AP targets L1TD1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16147 Product name: Recombinant human L1TD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 522-865 aa of BC111559 Sequence: KLVKHQVVHKTQEEEETAVPTSQGTGTPCLTLCLASPSKSLEMSHDEHKKHSHTNLSISTGVTKLKKTEEKKHRTLHTEELTSKEADLTEETEENLRSSVINSIREIKEEIGNLKSSHSGVLEIENSVDDLSSRMDILEERIDSLEDQIEEFSKDTMQMTKQIISKERQRDIEERSRSCNIRLIGIPEKESYENRAEDIIKEIIDENFAELKKGSSLEIVSACRVPSKIDEKRLTPRHILVKFWNSSDKEKIIRASRERREITYQGTRIRLTADLSLDTLDARSKWSNVFKVLLEKGFNPRILYPAKMAFDFRGKTKVFLSIEEFRDYVLHMPTLRELLGNNIP Predict reactive species |
| Full Name | LINE-1 type transposase domain containing 1 |
| Calculated Molecular Weight | 865 aa, 99 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC111559 |
| Gene Symbol | L1TD1 |
| Gene ID (NCBI) | 54596 |
| RRID | AB_2878878 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T7N2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells (hESCs) and has been considered as a marker for undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for L1TD1 antibody 21528-1-AP | Download protocol |
| IHC protocol for L1TD1 antibody 21528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The L1TD1 Polyclonal antibody (Cat# 21528-1-AP) has a total of 2 citations. These appear in Nature Communications (IF 18.1) and Advanced Science. The cited research fields include pluripotency and embryonic development (studying the microprotein PLUM encoded by Lin28b uORF as a cytoplasmic determinant of pluripotency) and RNA modification/epigenetics (profiling m⁶A readers that regulate human embryonic stem cell differentiation). Applications cited include WB, IP, and RIP in mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Microprotein PLUM encoded by Lin28b uORF is a cytoplasmic determinant of pluripotency and embryonic development | ||
Adv Sci (Weinh) Comprehensive Profiling of N(6)-methyladnosine (m(6)A) Readouts Reveals Novel m(6)A Readers That Regulate Human Embryonic Stem Cell Differentiation. |
