Tested Applications
| Positive WB detected in | A549 cells, rat brain tissue, mouse small intestine tissue, SGC-7901 cells, MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15707-1-AP targets L2HGDH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8382 Product name: Recombinant human L2HGDH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-351 aa of BC006117 Sequence: MVPALRYLVGACGRARGRFAGGSPGACGFASGRPRPLCGGSRSASTSSFDIVIVGGGIVGLASARALILRHPSLSIGVLEKEKDLAVHQTGHNSGVIHSGIYYKPESLKAKLCVQGAALLYEYCQQKGISYKQCGKLIVAVEQEEIPRLQALYEKGLQNGVPGLRLIQQEDIKKKEPYCRGLMAIDCPHTGIVDYRQVALSFAQDFQEAGGSVLTNFEVKGIEMAKESPSRSIDGMQYPIVIKNTKGEEIRCQYVVTCAGLYSDRISELSGCTPDPRIVPFRGDYLLLKPEKCYLVKGNIYPVPDSRFPFLGVHFTPRMDGSIWLGPNAVLAFKREGYRPFDFSATDVMDI Predict reactive species |
| Full Name | L-2-hydroxyglutarate dehydrogenase |
| Calculated Molecular Weight | 463aa,50 kDa; 441aa,49 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC006117 |
| Gene Symbol | L2HGDH |
| Gene ID (NCBI) | 79944 |
| RRID | AB_2133202 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9P8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for L2HGDH antibody 15707-1-AP | Download protocol |
| WB protocol for L2HGDH antibody 15707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-L2HGDH antibody (Cat# 15707-1-AP) has 19 citations across journals including Science, Cell Metabolism, Cancer Discovery, Nature Metabolism, Science Translational Medicine, Science Advances, Clinical Cancer Research, Oncogene, Cell Chemical Biology, Leukemia, Cell Reports, and others. Research fields span oncometabolite metabolism, epigenetics, cancer biology (renal, colorectal, glioblastoma, lung), T cell metabolism, embryo development, DNA repair, macrophage polarization, neurodegeneration, and diabetic kidney disease.
| Species | Application | Title |
|---|---|---|
Cell Metab Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.
| ||
Cancer Discov L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer. | ||
Sci Transl Med 2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity.
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Reviews
What customers say
Both reviewers rated the antibody 4 out of 5 stars for Western Blot. One user tested it on Renca mouse renal cancer cells at 1:1000 dilution, noting one non-specific band above the expected molecular weight, using 5% milk blocking and 3% BSA for primary antibody dilution. The other user tested it on mouse liver tissue at the same dilution after boiling in SDS sample buffer. Overall, users report good performance with minor non-specific binding in some conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anirban (Verified Customer) (12-05-2021) | 24 ug protein/lane. One non specific band observed above the KD band. 5% milk was used to block (1 hr incubation) and in secondary Ab (1/5000 dilution-45 minutes incubation). Primary Ab was diluted in 3% BSA (1.5 hrs incubation. All were done in TBST (0.1% Tween 20). Nitrocellulose membrane was used.
 |
Daniel (Verified Customer) (09-30-2019) | Tissue was boiled in SDS-sample buffer and separated by size on an SDS-PAGE.
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