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Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse brain tissue, mouse skeletal muscle tissue, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24307-1-AP targets LARGE in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19458 Product name: Recombinant human LARGE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-190 aa of BC126404 Sequence: SFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRMREVEEENRALRRQLSLAQGRAPSHRRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPVVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWM Predict reactive species |
| Full Name | like-glycosyltransferase |
| Calculated Molecular Weight | 756 aa, 88 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC126404 |
| Gene Symbol | LARGE |
| Gene ID (NCBI) | 9215 |
| RRID | AB_2879486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95461 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LARGE antibody 24307-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
