Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19333-1-AP targets LCA5 in WB, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5140 Product name: Recombinant human LCA5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 4-283 aa of BC050327 Sequence: RAGSPGTDQEREAGKHHYSYSSDFETPQSSGRSSLVSSSPASVRRKNPKRQTSDGQVHHQAPRKPSPKGLPNRKGVRVGFRSQSLNREPLRKDTDLVTKRILSARLLKINELQNEVSELQVKLAELLKENKSLKRLQYRQEKALNKFEDAENEISQLIFRHNNEITALKERLRKSQEKERATEKRVKDTESELFRTKFSLQKLKEISEARHLPERDDLAKKLVSAELKLDDTERRIKELSKNLELSTNSFQRQLLAERKRAYEAHDENKVLQKEVQRLYH Predict reactive species |
| Full Name | Leber congenital amaurosis 5 |
| Calculated Molecular Weight | 693 aa, 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC050327 |
| Gene Symbol | LCA5 |
| Gene ID (NCBI) | 167691 |
| RRID | AB_2878576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LCA5 (Leber congenital amaurosis 5), also named as C6orf152 or Lebercilin, is a 697 amino acids protein. LCA5 is widely expressed at the microtubules, centrosome, and primary cilia. Recent study showed that LCA5 was involved in the cause of congenital and early-onset retinal dystrophies (PMID: 24144451). The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LCA5 antibody 19333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Mol Ther Amelioration of Neurosensory Structure and Function in Animal and Cellular Models of a Congenital Blindness. | ||
Hum Mol Genet Disruption of the Retinitis Pigmentosa 28 gene Fam161a in mice affects photoreceptor ciliary structure and leads to progressive retinal degeneration. | ||
Invest Ophthalmol Vis Sci Treatment Potential for LCA5-Associated Leber Congenital Amaurosis. | ||
J Proteomics Tissue- and isoform-specific protein complex analysis with natively processed bait proteins. | ||
EMBO J Glutamylation imbalance impairs the molecular architecture of the photoreceptor cilium |
