Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, Ramos cells |
| Positive IHC detected in | human breast cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16024-1-AP targets LETM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8861 Product name: Recombinant human LETM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 391-739 aa of BC021208 Sequence: EDRLRGQLKQWLDLHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSEVAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETLKDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDVHISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVAEVKS Predict reactive species |
| Full Name | leucine zipper-EF-hand containing transmembrane protein 1 |
| Calculated Molecular Weight | 739 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC021208 |
| Gene Symbol | LETM1 |
| Gene ID (NCBI) | 3954 |
| RRID | AB_2136505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95202 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LETM1 antibody 16024-1-AP | Download protocol |
| IHC protocol for LETM1 antibody 16024-1-AP | Download protocol |
| WB protocol for LETM1 antibody 16024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-LETM1 antibody (Cat# 16024-1-AP) has 21 citations published across 17 journals, including Nature, Nature Communications, Science Advances, PNAS, Cell Reports, and Theranostics. Research fields span mitochondrial calcium homeostasis, cardiac physiology and pathology, energy metabolism, cancer biology, and neurology. Applications are predominantly Western blot, with some immunofluorescence, co-immunoprecipitation, and immunoprecipitation studies. Species studied include mouse, human, rat, and unspecified.
| Species | Application | Title |
|---|---|---|
Nature The mitochondrial Na(+)/Ca(2+) exchanger is essential for Ca(2+) homeostasis and viability. | ||
Nat Commun FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes | ||
Nat Commun Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy. | ||
Nat Commun Loss of TMEM65 in mice causes mitochondrial disease mediated by mitochondrial Ca(2). | ||
Theranostics Nitric oxide-primed engineered extracellular vesicles restore bioenergetics in acute kidney injury via mitochondrial transfer | ||
Genes Dis Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma
|
Reviews
What customers say
The product has received 1 review with a 5-star rating. The reviewer used it for Western Blot on mouse heart tissue at a 1:1000 dilution and described the antibody as "easy to expose," indicating straightforward and reliable performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
SITING (Verified Customer) (08-31-2020) | easy to expose
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