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Tested Applications
| Positive WB detected in | mouse small intestine tissue, rat small intestine tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
12849-2-AP targets Galectin 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3979 Product name: Recombinant human LGALS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-130 aa of BC029063 Sequence: ELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTFESDKFKVKLPDGHELTFPNRLGHSHLSYLSVRGGFNMSSFKLKE Predict reactive species |
| Full Name | lectin, galactoside-binding, soluble, 2 |
| Calculated Molecular Weight | 132 aa, 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC029063 |
| Gene Symbol | Galectin 2 |
| Gene ID (NCBI) | 3957 |
| RRID | AB_2136753 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05162 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Galectin 2 antibody 12849-2-AP | Download protocol |
| WB protocol for Galectin 2 antibody 12849-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin. | ||
J Gastroenterol Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer. | ||
Oncotarget Galectin signatures contribute to the heterogeneity of breast cancer and provide new prognostic information and therapeutic targets. |
