Tested Applications
| Positive WB detected in | mouse small intestine tissue, rat small intestine tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12849-2-AP targets Galectin 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3979 Product name: Recombinant human LGALS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-130 aa of BC029063 Sequence: ELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTFESDKFKVKLPDGHELTFPNRLGHSHLSYLSVRGGFNMSSFKLKE Predict reactive species |
| Full Name | lectin, galactoside-binding, soluble, 2 |
| Calculated Molecular Weight | 132 aa, 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC029063 |
| Gene Symbol | Galectin 2 |
| Gene ID (NCBI) | 3957 |
| RRID | AB_2136753 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05162 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Galectins are a class of carbohydrate-binding proteins implicated in multiple physiological processes including inflammation, immune responses, cell migration, autophagy, and signal transduction (PMID: 29717004). Galectin-2 (Gal-2, also known as LGALS2) is a monocyte-derived carbohydrate-binding lectin; its upregulation correlates with reduced collateral arteriogenesis in patients with obstructive coronary artery disease (PMID: 25884209). Additionally, Gal-2 may interact with T cells, particularly in the setting of inflammatory bowel disease (PMID: 15356130).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Galectin 2 antibody 12849-2-AP | Download protocol |
| WB protocol for Galectin 2 antibody 12849-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Galectin 2 Polyclonal antibody (Cat# 12849-2-AP) has a total of 3 citations. These appear in Oncotarget (2 papers) and J Gastroenterol (1 paper). The cited research fields include cancer biology, specifically gastric cancer lymph node metastasis, breast cancer heterogeneity and prognosis, and design of peptidic inhibitors targeting the galectin dimer interface. All citations involve human samples, with applications in WB and IHC.
| Species | Application | Title |
|---|---|---|
Oncotarget Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin. | ||
J Gastroenterol Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer. | ||
Oncotarget Galectin signatures contribute to the heterogeneity of breast cancer and provide new prognostic information and therapeutic targets. |
