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LILRA2 Polyclonal antibody

LILRA2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11461-1-AP

Synonyms

CD85H, ILT 1, ILT1, LILRA2, LIR 7, LIR7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LILRA2 Polyclonal antibody (11461-1-AP)

    WB analysis using 11461-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11461-1-AP (LILRA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using LILRA2 Polyclonal antibody (11461-1-AP)

    WB analysis of pig spleen using 11461-1-AP

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 11461-1-AP (LILRA2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LILRA2 Polyclonal antibody (11461-1-AP)

    IHC staining of human tonsillitis using 11461-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11461-1-AP (LILRA2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LILRA2 Polyclonal antibody (11461-1-AP)

    IHC staining of human tonsillitis using 11461-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11461-1-AP (LILRA2 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, pig spleen tissue, HepG2 cells, THP-1 cells, human peripheral blood leukocytes
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11461-1-AP targets LILRA2 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LILRA2 fusion protein Ag2003
Full Name leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2
Calculated Molecular Weight 466 aa, 51 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC017412
Gene Symbol LILRA2
Gene ID (NCBI) 11027
RRIDAB_2136370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LILRA2 antibody 11461-1-APDownload protocol
IHC protocol for LILRA2 antibody 11461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Free Radic Biol Med

Altered proximal tubule fatty acid utilization, mitophagy, fission and supercomplexes arrangement in experimental Fanconi syndrome are ameliorated by sulforaphane-induced mitochondrial biogenesis.

Authors - Alfredo Briones-Herrera
View Article
mouseWB

Phytomedicine

Epigallocatechin gallate and theaflavins independently alleviate cyclophosphamide-induced ovarian damage by inhibiting the overactivation of primordial follicles and follicular atresia.

Authors - Qian Chen
View Article