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ERGIC-53 Polyclonal antibody
ERGIC-53 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13364-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, human brain tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse heart tissue, mouse spleen tissue, rat heart tissue, rat spleen tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
| IHC | See 1 publications below |
| IF | See 12 publications below |
Product Information
13364-1-AP targets ERGIC-53 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ERGIC-53 fusion protein Ag4183 |
| Full Name | lectin, mannose-binding, 1 |
| Calculated Molecular Weight | 510 aa, 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC032330 |
| Gene Symbol | LMAN1 |
| Gene ID (NCBI) | 3998 |
| RRID | AB_2135994 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERGIC-53 (also known as LMAN1 or MR60) is a membrane mannose-specific lectin that selectively transports its cargo proteins from ER to ER-Golgi intermediate compartment (ERGIC) and Golgi, functioning as a cargo transport receptor for glycoproteins (PMID: 24664723; 10559958). Mutations in ERGIC-53 cause combined deficiency of coagulation factors V and VIII (PMID: 9546392).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERGIC-53 antibody 13364-1-AP | Download protocol |
| IHC protocol for ERGIC-53 antibody 13364-1-AP | Download protocol |
| IF protocol for ERGIC-53 antibody 13364-1-AP | Download protocol |
| IP protocol for ERGIC-53 antibody 13364-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis | ||
Nat Commun ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling | ||
Nat Commun Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH pitna (Verified Customer) (05-26-2021) | for Ergic Immunoprecipitation validation for human brain
 |
FH Boyan (Verified Customer) (02-19-2020) | For WB, this antibody could detect endogenous Ergic53 with a very clean band.
|
