LMNB1 Antibody
Rabbit Polyclonal
Catalog number: 12987-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse, rat |
| Positive WB detected in | HeLa cells, HepG2 cells, human brain tissue, human kidney tissue, Jurkat cells, K-562 cells, mouse brain tissue, mouse eye tissue, mouse kidney tissue, NIH/3T3 cells, Y79 cells |
| Positive IHC detected in | human breast cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-LMNB1 (12987-1-AP for IP and Detection) with HeLa cell lysate.
HeLa cells were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human breast cancer using 12987-1-AP(LMNB1 Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | LMNB1 fusion protein ag3631 | Full name | lamin B1 |
|
Calculated molecular weight |
66kd |
Observed molecular
weight |
66kd |
|
GenBank accession number |
BC012295 | Gene ID (NCBI) | 4001 |
| Gene symbol | LMNB1 | Synonyms | ADLD; LMN; LMN2; LMNB; MGC111419 |
Background
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed. |
