LMNB1 AntibodyMouse Monoclonal
|Tested applications||ELISA, WB, IF|
|Species specificity||human, mouse, rat; other species not tested.|
|Positive IF detected in||HepG2 cells|
|Recommended dilution||WB: 1:20000-1:100000|
IP = Immunoprecipitation
- Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-Ig) at dilution 1:20000.
- Western blot of Hela cell with anti-LMNB1 (66095-1-Ig) at various dilutions.
- Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 66095-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
|Source||Mouse||Purification method||Protein G purification|
|Isotype||IgG1||Storage||PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||LMNB1 fusion protein ag20522||Full name||lamin B1|
|BC012295||Gene ID (NCBI)||4001|
|Gene symbol||LMNB1||Synonyms||ADLD; LMN; LMN2; LMNB; MGC111419|
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.