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- KD/KO Validated
LMOD3 Polyclonal antibody
LMOD3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14948-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 13 publications below |
| IHC | See 3 publications below |
| IF | See 5 publications below |
Product Information
14948-1-AP targets LMOD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LMOD3 fusion protein Ag6758 |
| Full Name | leiomodin 3 (fetal) |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC039202 |
| Gene Symbol | LMOD3 |
| Gene ID (NCBI) | 56203 |
| RRID | AB_2136690 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LMOD3 antibody 14948-1-AP | Download protocol |
| IHC protocol for LMOD3 antibody 14948-1-AP | Download protocol |
| IP protocol for LMOD3 antibody 14948-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle. | ||
J Clin Invest Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy. | ||
J Clin Invest Severe myopathy in mice lacking the MEF2/SRF-dependent gene leiomodin-3.
| ||
J Clin Invest Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy. | ||
J Clin Invest KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy. | ||
Proc Natl Acad Sci U S A HSPB7 is indispensable for heart development by modulating actin filament assembly. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tongbin (Verified Customer) (08-25-2020) | This antibody is very specific. I had a single Lmod3 band at around 70kD. Western blot results of this antibody had been published in our PNAS paper (PMID: 29078393)
 |
