Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
17169-1-AP targets LOH12CR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10695 Product name: Recombinant human LOH12CR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-196 aa of BC013668 Sequence: MGSEQSSEAESRPNDLNSSVTPSPAKHRAKMDDIVVVAQGSQASRNVSNDPDVIKLQEIPTFQPLLKGLLSGQTSPTNAKLEKLDSQQVLQLCLRYQDHLHQCAEAVAFDQNALVKRIKEMDLSVETLFSFMQERQKRYAKYAEQIQKVNEMSAILRRIQMGIDQTVPLLDRLNSMLPEGERLEPFSMKPDRELRL Predict reactive species |
| Full Name | loss of heterozygosity, 12, chromosomal region 1 |
| Calculated Molecular Weight | 196 aa, 22 kDa |
| Observed Molecular Weight | 17-22 kDa |
| GenBank Accession Number | BC013668 |
| Gene Symbol | LOH12CR1 |
| Gene ID (NCBI) | 118426 |
| RRID | AB_2137150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969J3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LOH12CR1, also named as BORCS5, is part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. It may indirectly play a role in cell spreading and motility. LOH12CR1 has two isoforms with MW 17 kDa and 22 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
| IHC protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
| WB protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. | ||
Cell Rep Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes | ||
Cell Rep Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.
| ||
Cell Genom Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders | ||
Nat Neurosci Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration |
