Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
18035-1-AP targets LONP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12650 Product name: Recombinant human LONP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 503-852 aa of BC093910 Sequence: DRMEIIQVPGYTQEEKIEIAHRHLIPKQLEQHGLTPQQIQIPQVTTLDIITRYTREAGVRSLDRKLGAICRAVAVKVAEGQHKEAKLDRSDVTEREGCREHILEDEKPESISDTTDLALPPEMPILIDFHALKDILGPPMYEMEVSQRLSQPGVAIGLAWTPLGGEIMFVEASRMDGEGQLTLTGQLGDVMKESAHLAISWLRSNAKKYQLTNAFGSFDLLDNTDIHLHFPAGAVTKDGPSAGVTIVTCLASLFSGRLVRSDVAMTGEITLRGLVLPVGGIKDKVLAAHRAGLKQVIIPRRNEKDLEGIPGNVRQDLSFVTASCLDEVLNAAFDGGFTVKTRPGLLNSKL Predict reactive species |
| Full Name | lon peptidase 2, peroxisomal |
| Calculated Molecular Weight | 852 aa, 95 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC093910 |
| Gene Symbol | LONP2 |
| Gene ID (NCBI) | 83752 |
| RRID | AB_2878485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86WA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LONP2 (Lon Peptidase 2, Peroxisomal) is an important ATP-dependent protease. It is primarily located in peroxisomes and is responsible for maintaining the proteostasis of this organelle. By degrading misfolded proteins and selectively regulating metabolic enzymes, LONP2 plays a central role in maintaining peroxisomal proteostasis and metabolic balance. Dysfunction of LONP2 is closely related to a variety of human diseases, including peroxisomal disorders, cancer, and neurodegenerative diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LONP2 antibody 18035-1-AP | Download protocol |
| IHC protocol for LONP2 antibody 18035-1-AP | Download protocol |
| WB protocol for LONP2 antibody 18035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
