Tested Applications
| Positive WB detected in | HT-29 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17905-1-AP targets LPCAT4 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12281 Product name: Recombinant human LPCAT4 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 175-524 aa of BC092463 Sequence: PASRRRVVEEVRRRATSGGKWPQVLFFPEGTCSNKKALLKFKPGAFIAGVPVQPVLIRYPNSLDTTSWAWRGPGVLKVLWLTASQPCSIVDVEFLPVYHPSPEESRDPTLYANNVQRVMAQALGIPATECEFVGSLPVIVVGRLKVALEPQLWELGKVLRKAGLSAGYVDAGAEPGRSRMISQEEFARQLQLSDPQTVAGAFGYFQQDTKGLVDFRDVALALAALDGGRSLEELTRLAFELFAEEQAEGPNRLLYKDGFSTILHLLLGSPHPAATALHAELCQAGSSQGLSLCQFQNFSLHDPLYGKLFSTYLRPPHTSRGTSQTPNASSPGNPTALANGTVQAPKQKGD Predict reactive species |
| Full Name | lysophosphatidylcholine acyltransferase 4 |
| Calculated Molecular Weight | 524 aa, 57 kDa |
| Observed Molecular Weight | 50-57 kDa |
| GenBank Accession Number | BC092463 |
| Gene Symbol | LPCAT4 |
| Gene ID (NCBI) | 254531 |
| RRID | AB_2140478 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q643R3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LPCAT4, also known as lysophosphatidylcholine acyltransferase 4, is an enzyme that plays a crucial role in lipid metabolism and membrane remodeling It is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family and is involved in the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction. The protein is predicted to be located in the membrane and is classified as a metabolic protein and enzyme. It is involved in biological processes such as lipid biosynthesis, lipid metabolism, phospholipid biosynthesis, and phospholipid metabolism. .
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LPCAT4 antibody 17905-1-AP | Download protocol |
| WB protocol for LPCAT4 antibody 17905-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LPCAT4 Polyclonal antibody (Cat# 17905-1-AP) has 10 citations published in journals including J Hepatol, J Clin Invest, Free Radic Biol Med, Nat Prod Bioprospect, J Lipid Res, Neurochem Res, FASEB J, Front Biosci, and Nutrition. Research fields span hepatoma progression, leukemia metabolism, ferroptosis, prostate cancer, phospholipid biosynthesis, neurodegeneration, insulin secretion, glioma, lung adenocarcinoma, and brain iron/fatty acid metabolism.
| Species | Application | Title |
|---|---|---|
J Hepatol Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression. | ||
J Clin Invest Leukemia-expanded splenic CD81+ erythroblasts potentiate disease progression in mice by reshaping leukemic cell metabolism. | ||
Free Radic Biol Med Role of ferroptosis mediated by abnormal membrane structure in DEHP-induced reproductive injury | ||
Nat Prod Bioprospect Targeting Hsp70 triggers ferroptosis: a novel anti-cancer mechanism of a marine natural product in prostate cancer. | ||
J Lipid Res LPLAT10/LPEAT2 produces atypical phospholipids with an unsaturated FA at the sn-1 position.
| ||
Neurochem Res iPLA2β Knockout Mouse, a Genetic Model for Progressive Human Motor Disorders, Develops Age-Related Neuropathology. |
