Filter: AllWBIPIHCIF/ICC

WB analysis using 25045-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 25045-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 25045-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 25045-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 25045-1-AP
IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.
IHC staining of human small intestine using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 25045-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 25045-1-AP
Immunofluorescent analysis of HeLa cells using 25045-1-AP (LPP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

LPP Polyclonal antibody

Name: LPP Polyclonal antibody
Brand: Proteintech
SKU: 25045-1-AP

LPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 25045-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon, mouse heart, rat heart
Positive IP detected in	HeLa cells
Positive IHC detected in	human small intestine tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

25045-1-AP targets LPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19205 Product name: Recombinant human LPP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC130584 Sequence: MSHPSWLPPKSTGEPLGHVPARMETTHSFGNPSISVSTQQPPKKFAPVVAPKPKYNPYKQPGGEGDFLPPPPPPLDDSSALPSISGNFPPPPPLDEEAFKVQGNPGGKTLEERRSSLDAEIDSLTSILADLECSSPYKPRPPQSSTGSTASPPVSTPVTGHKRMVIPNQPPLTATKKSTLKPQPAPQAGPIPVAPIGTLKPQPQPVPASYTTASTSSRPTFNVQVKSAQPSPHYMAAPSSGQIYGSGPQGYNTQPVPVSGQCPPPSTRGGMDYAYIPPPGLQPEPGYGYAPNQGRYYEGYYAAGPGYGGRNDSDPTYGQQGHPNTWKREPGYTPPGAGNQNPPGMYPVTG Predict reactive species
Full Name	LIM domain containing preferred translocation partner in lipoma
Calculated Molecular Weight	612 aa, 66 kDa
Observed Molecular Weight	70-75 kDa
GenBank Accession Number	BC130584
Gene Symbol	LPP
Gene ID (NCBI)	4026
RRID	AB_2879869
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q93052
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).

Protocols

Product Specific Protocols
IF protocol for LPP antibody 25045-1-AP	Download protocol
IHC protocol for LPP antibody 25045-1-AP	Download protocol
IP protocol for LPP antibody 25045-1-AP	Download protocol
WB protocol for LPP antibody 25045-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF	Cell Death Dis UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer Authors - Kanghua Xiao View Article