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LSG1 Polyclonal antibody

LSG1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17750-1-AP

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

EC:3.6.1.-, hLsg1, LSG 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of HeLa using 17750-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17750-1-AP (LSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 17750-1-AP
IP result of anti-LSG1 (IP:17750-1-AP, 4ug; Detection:17750-1-AP 1:500) with HeLa cells lysate 1400 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat testis using 17750-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17750-1-AP (LSG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat testis using 17750-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17750-1-AP (LSG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 17750-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 17750-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	rat testis tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17750-1-AP targets LSG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12016 Product name: Recombinant human LSG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 260-658 aa of BC068500 Sequence: DRQSNTTEFGHSSFDQAEISHSESEHLPARDSPSLSENPTTDEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQDWKESSTADSEARSRKTPQKRQIHNFSHLVSKQELLELFKELHTGRKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGHTKHFQTLYVEPGLCLCDCPGLVMPSFVSTKAEMTCSGILPIDQMRDHVPPVSLVCQNIPRHVLEATYGINIITPREDEDPHRPPTSEELLTAYGYMRGFMTAHGQPDQPRSARYILKDYVSGKLLYCHPPPGRDPVTFQHQHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQAVMGYKPGSGVVTASTASSENGAGKPWKKHGNRNKKEKSRRLYKHLDM Predict reactive species
Full Name	large subunit GTPase 1 homolog (S. cerevisiae)
Calculated Molecular Weight	658 aa, 75 kDa
Observed Molecular Weight	75 kDa
GenBank Accession Number	BC068500
Gene Symbol	LSG1
Gene ID (NCBI)	55341
RRID	AB_2281443
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H089
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for LSG1 antibody 17750-1-AP	Download protocol
IP protocol for LSG1 antibody 17750-1-AP	Download protocol
WB protocol for LSG1 antibody 17750-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

LSG1 Polyclonal antibody (Cat# 17750-1-AP) has 4 total citations published in EMBO Molecular Medicine, Aging Cell, Molecular and Cellular Biology, and bioRxiv. The associated research fields include inflammasome-mediated skin inflammation, ribosome biogenesis and endoplasmic reticulum stress, vesicle-associated membrane protein interactions, and endosome-to-TGN trafficking in AAV transduction.

Species	Application	Title
		EMBO Mol Med Portimine A toxin causes skin inflammation through ZAKα-dependent NLRP1 inflammasome activation Authors - Léana Gorse View Article
human	WB,IF	Aging Cell Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence. Authors - Asimina Pantazi KD Validated View Article
human	WB	Mol Cell Biol The Ribosome Assembly Factor LSG1 Interacts with Vesicle-Associated Membrane Protein-Associated Proteins (VAPs) Authors - Putri Sutjita View Article
human	WB	bioRxiv TBC1D23-AAVR Interaction Drives Endosome-to-TGN Trafficking Required for rAAV Transduction. Authors - Xiujuan Zhang View Article