Tested Applications
| Positive WB detected in | HeLa cells, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10259-1-AP targets LSM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0232 Product name: Recombinant human LSM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-133 aa of BC001767 Sequence: MNYMPGTASLIEDIDKKHLVLLRDGRTLIGFLRSIDQFANLVLHQTVERIHVGKKYGDIPRGIFVVRGENVVLLGEIDLEKESDTPLQQVSIEEILEEQRVEQQTKLEAEKLKVQALKDRGLSIPRADTLDEY Predict reactive species |
| Full Name | LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae) |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | BC001767 |
| Gene Symbol | LSM1 |
| Gene ID (NCBI) | 27257 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lsm1 is a component of the Lsm1-7 complex involved in cytoplasmic mRNA degradation. Lsm1 is a key subunit that determines the RNA-binding properties of this complex. The normal RNA-binding activity of this complex is crucial for mRNA decay and 3'-end protection in vivo and requires the intact Sm-domain of Lsm1. LSM1 localizes to cytoplasmic processing bodies (P bodies) involved in mRNA storage, processing, regulation, and degradation and is required for P-body formation. LSM1 proteins facilitate the formation of U4/U6 RNA duplexes in vitro and may play a role in U4/U6 snRNP formation.
