Tested Applications
| Positive WB detected in | HT-1376 cells, PC-3 cells, HeLa cells, HepG2 cells, mouse testis tissue, human brain tissue, mouse pancreas tissue, mouse liver tissue, SH-SY5Y cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13715-1-AP targets LSS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4655 Product name: Recombinant human LSS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 382-732 aa of BC035638 Sequence: NGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRLSQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETLTQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWGEDFESCEERRYVQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIFPIWALGRFSQLYPERALAGHP Predict reactive species |
| Full Name | lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) |
| Calculated Molecular Weight | 732 aa, 83 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC035638 |
| Gene Symbol | LSS |
| Gene ID (NCBI) | 4047 |
| RRID | AB_10597096 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48449 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LSS antibody 13715-1-AP | Download protocol |
| IHC protocol for LSS antibody 13715-1-AP | Download protocol |
| IP protocol for LSS antibody 13715-1-AP | Download protocol |
| WB protocol for LSS antibody 13715-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LSS Polyclonal antibody (Cat# 13715-1-AP) has 26 citations across journals including Nature Communications, Cell Host & Microbe, Neuron, Oncogene, Cell Reports, Signal Transduction and Targeted Therapy, Cancer Letters, Journal of Biological Chemistry, and others. Research fields span cholesterol biosynthesis and metabolism, cancer (colorectal, breast, hepatocellular, prostate, esophageal), cardiovascular disease, neurology, immunology, dermatology, ophthalmology, and endocrinology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy. | ||
Cell Host Microbe BCAA-producing Clostridium symbiosum promotes colorectal tumorigenesis through the modulation of host cholesterol metabolism | ||
Nat Commun Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Redox Biol ALOX15B controls macrophage cholesterol homeostasis via lipid peroxidation, ERK1/2 and SREBP2 | ||
Cancer Lett SQLE amplification accelerates esophageal squamous cell carcinoma tumorigenesis and metastasis through oncometabolite 2,3-oxidosqualene repressing Hippo pathway |
